Artículo

CRISPR-Cas12/Cas13: Bibliometric analysis and systematic review of its application in infectious disease detection

Resumen

Background Infectious diseases impose a significant burden on the global public health and economy, resulting in an estimated 15 million deaths out of 57 million annually worldwide. This study examines the current state of CRISPR-Cas12/Cas13 research, focusing on its applications in infectious disease detection and its evolutionary trajectory. Methods A bibliometric analysis and systematic review were conducted by retrieving CRISPR-Cas12/Cas13-related articles published between January 1, 2015 to December 31, 2022, from the Web of Science database. The research protocol was registered with International Platform of Registered Systematic Review and Meta-analysis Protocols (INPLASY202380062). Results Our search identified 1987 articles, of which, 1856 were included in the bibliometric analysis and 445 were used in qualitative analysis. The study reveals a substantial increase in scientific production on CRISPR-Cas12/Cas13, with an annual growth rate of 104.5%. The United States leads in the number of published articles. The systematic review identified 580 different diagnostic assays targeting 170 pathogens, with SARS-CoV-2 dominating with 158 assays. Recombinase polymerase amplification (RPA)/reverse transcription-RPA (RT-RPA) emerged as the predominant amplification method, while lateral flow assay was the most common readout method. Approximately 72% of the diagnostic assays developed are suitable for point-of-care testing. Conclusion The rapid increase in research on CRISPR-Cas12/Cas13 between 2015 and 2022 suggests promising potential for advancements in infectious disease diagnosis. Given the numerous advantages of CRISPR-Cas technology for disease detection over other methods, and the dedicated efforts of scientists from around the world, it is reasonable to anticipate that CRISPR-Cas technology may emerge as a formidable alternative, offering the possibility of expedited point-of-care testing in the not-too-distant future.
Leta, Samson (37057669800); Chibssa, Tesfaye Rufael (56293388800); Paeshuyse, Jan (6506452859)
CRISPR-Cas12/Cas13: Bibliometric analysis and systematic review of its application in infectious disease detection
2024
10.1016/j.jiph.2024.03.003
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85188417589&doi=10.1016%2fj.jiph.2024.03.003&partnerID=40&md5=beacd26324c15958e87c01018d45d1d9
Laboratory of Host Pathogen Interaction in Livestock, Division of Animal and Human Health Engineering, Department of Biosystems, KU Leuven, Leuven, 3001, Belgium; Department of Biomedical Sciences, College of Veterinary Medicine and Agriculture, Addis Ababa University, P.O. Box 34, Bishoftu, Ethiopia; Animal Health Institute (AHI), P.O. Box 04, Sebeta, Ethiopia
All Open Access; Gold Open Access
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